COC (Chain of Custody)
A documented process that tracks the handling of a drug test specimen from collection through final reporting to ensure its integrity.
Key Facts
- Tracks specimen from collection through testing to reporting
- Federal CCF form required for all DOT drug tests
- Any break in custody can invalidate the test result
- Collector, lab, and MRO each sign the custody form
The chain of custody (COC) form accompanies every drug test specimen from the moment of collection through laboratory analysis and result reporting. The Federal Drug Testing Custody and Control Form (CCF) records: donor identification, specimen temperature check (within 90-100°F within 4 minutes of collection), collector certification, laboratory processing, and MRO review. Any break in the chain of custody can invalidate the test result. The COC ensures that the specimen tested belongs to the identified donor and has not been tampered with.
Drug Testing Procedures Compared
Key procedural elements that ensure drug test integrity and accuracy.
| Type | What It Covers | Regulation | Key Requirement | If Violated |
|---|---|---|---|---|
| Chain of Custody | Specimen tracking | 49 CFR Part 40 | Unbroken documentation | Test may be invalidated |
| CCF Form | Collection documentation | 49 CFR Part 40 | Multi-part form for all DOT tests | Test cancelled |
| Split Specimen | Retest option | 49 CFR Part 40 | Bottle A (30 mL) + Bottle B (15 mL) | Test cancelled if B negative |
| Cutoff Levels | Positive/negative threshold | SAMHSA guidelines | Two-stage: screen then confirm | False positives/negatives |
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