49 CFR Part 40

Federal regulation governing procedures for DOT workplace drug and alcohol testing programs across all DOT agencies.

Key Facts

  • Governs all DOT workplace drug and alcohol testing procedures
  • Applies to all DOT agencies: FMCSA, FAA, FRA, FTA, PHMSA
  • Covers specimen collection, lab testing, MRO review, and SAP process
  • Updated in 2023 to allow oral fluid testing

49 CFR Part 40 ("Procedures for Transportation Workplace Drug and Alcohol Testing Programs") establishes the uniform procedures for all DOT drug and alcohol testing. It covers: specimen collection procedures, laboratory testing standards, Medical Review Officer (MRO) verification process, Substance Abuse Professional (SAP) evaluation requirements, employer responsibilities, and employee rights. Part 40 applies to all DOT agencies (FMCSA, FAA, FRA, FTA, PHMSA) and ensures consistent testing practices across the transportation industry.

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