Negative Drug Test Result

A drug test result indicating no controlled substance was detected at or above the established cutoff level.

Key Facts

  • No controlled substance detected at or above cutoff levels
  • MRO reviews and releases the result to the employer
  • Can still be flagged as "negative-dilute" if specimen is dilute
  • Employee cleared for safety-sensitive duties
  • Result reported within 24–48 hours typically

A negative drug test result means the specimen was tested and no controlled substances were identified at or above the screening and confirmation cutoff concentrations. The MRO reviews the result and releases it to the employer (via the DER). A negative result clears the employee to begin or continue safety-sensitive functions. However, a negative result with dilute specimen markers (negative-dilute) may prompt the employer to request a single retest. Under DOT regulations, the employer has the option — but not the obligation — to retest for negative-dilute results.

Drug Test Results & Specimen Issues Compared

Understanding the different outcomes and specimen-related issues in drug testing.

TypeMeaningReported ByNext StepsMRO Role
PositiveConfirmed presence above cutoffLaboratory → MROMRO interview, then report to employerVerify with donor — medical explanation?
NegativeNo substances detected above cutoffLaboratory → MROEmployee clearedReview and release result
DiluteCreatinine 2–20 mg/dL & SG 1.0001–1.0029Laboratory → MROEmployer may request retest (one time)Report as negative-dilute or positive-dilute
Shy Bladder / Shy LungUnable to provide sufficient specimenCollectorMedical evaluation within 5 daysEvaluate medical explanation

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