Reasonable suspicion testing is triggered by trained supervisor observations of appearance, behavior, speech, or body odor consistent with drug use or alcohol misuse. Under DOT regulations (49 CFR 382.307), the supervisor must have received at least 60 minutes of training on alcohol misuse indicators and 60 minutes on drug use indicators. The supervisor must document their observations before the test. For alcohol, the test must be administered within 2 hours if possible and no later than 8 hours after the observation. For drugs, there is no specific time limit, but testing should occur as soon as possible.