DOT Drug Test (10-Panel): Employer's Quick Guide
Everything employers need to know about DOT 10-Panel — who needs it, what's included, and how to find a provider through BlueHive.

At a glance
- Turnaround
- Negative: 24-48 hours | Positive: 3-5 days
- Typical cost
- $60–$120
What Is DOT 10-Panel?
DOT-mandated 10-panel drug test for safety-sensitive employees in transportation industries.
The DOT 10-Panel Drug Test is the standard urine drug test required by the Department of Transportation for safety-sensitive employees. The test screens for marijuana (THC), cocaine, amphetamines (including methamphetamine and MDMA), opioids (including codeine, morphine, heroin, hydrocodone, hydromorphone, oxycodone, and oxymorphone), and phencyclidine (PCP).
Who Needs This Service?
- Commercial motor vehicle drivers
- Pipeline workers
- Railroad employees
- Aviation personnel
- Transit workers
What's Included
A typical DOT 10-Panel appointment includes:
- SAMHSA-certified laboratory testing
- Certified collector network
- Medical Review Officer (MRO) review
- Electronic Chain of Custody
- Results within 24-48 hours
Turnaround time: Negative: 24-48 hours | Positive: 3-5 days
Typical cost: $60–$120
How It Works
- Order — Employer orders DOT drug test through BlueHive.
- Collection — Employee visits certified collection site for observed/unobserved collection.
- Testing — Specimen tested at SAMHSA-certified laboratory.
Related Services
Ready to get started? Find a provider for DOT 10-Panel or learn more about this service.
How DOT 10-Panel works, step by step
- 1
Order
Employer orders DOT drug test through BlueHive.
- 2
Collection
Employee visits certified collection site for observed/unobserved collection.
- 3
Testing
Specimen tested at SAMHSA-certified laboratory.
- 4
MRO Review
MRO verifies and reports results to employer.
Regulatory snapshot
49 CFR Part 40 DOT drug testing procedures; SAMHSA-certified laboratory required; MRO review mandatory
Track state-by-state changes in Compliance WatchFrequently asked questions
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