DOT Drug Test - 5 Panel: Employer's Quick Guide
Everything employers need to know about DOT 5-Panel — who needs it, what's included, and how to find a provider through BlueHive.
At a glance
- Turnaround
- 24-48 hours (negative)
- Typical cost
- $45–$75
- Certification
- DOT/SAMHSA Compliant
- CPT code
- 80305
What Is DOT 5-Panel?
The standard DOT-mandated 5-panel urine drug test required for safety-sensitive transportation employees.
The DOT 5-Panel Drug Test is the federally-mandated drug screening required for all safety-sensitive employees in DOT-regulated industries. This test screens for five categories of drugs: marijuana, cocaine, opiates, amphetamines/methamphetamines, and phencyclidine (PCP).
Who Needs This Service?
- CDL drivers and trucking companies
- Airlines and aviation workers
- Pipeline operators
- Railroad employees
- Transit authorities
What's Included
A typical DOT 5-Panel appointment includes:
- HHS-certified laboratory testing
- Strict chain of custody procedures
- MRO (Medical Review Officer) review included
- Electronic CCF (Custody and Control Form)
- Results typically within 24-48 hours
Turnaround time: 24-48 hours (negative)
Typical cost: $45–$75
How It Works
- Order the Test — Request a DOT drug test through BlueHive for your employee.
- Collection — Employee provides a urine specimen at a certified collection site with proper chain of custody.
- Laboratory Analysis — Specimen is analyzed at an HHS-certified laboratory.
Related Services
Ready to get started? Find a provider for DOT 5-Panel or learn more about this service.
How DOT 5-Panel works, step by step
- 1
Order the Test
Request a DOT drug test through BlueHive for your employee.
- 2
Collection
Employee provides a urine specimen at a certified collection site with proper chain of custody.
- 3
Laboratory Analysis
Specimen is analyzed at an HHS-certified laboratory.
- 4
MRO Review
A Medical Review Officer reviews results and contacts the employee if needed before finalizing.
Regulatory snapshot
49 CFR Part 40; HHS laboratory certification required; MRO review mandatory; Clearinghouse reporting for positives
Track state-by-state changes in Compliance WatchFrequently asked questions
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Content Developer
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Chris Davis is a content developer at BlueHive Health with a background in healthcare operations, medical billing, and small-business management. He writes plain-English guides on OSHA, DOT, and PHMSA compliance for HR and safety leaders.
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